INTELBIOCOMP

Fabrication and investigation of new hybrid scaffolds with controlled porous hierarchy for bone tissue engineering: Scaffold characterization with synchrotron X-rays

One of the largest application areas of biomaterials is in the medical treatment of osteoporosis and bone cancer. A major challenge for the development of biocompatible materials for bone-tissue engineering is the production of clinically useable scaffolds and implants which can be obtained “on-demand”. INTELBIOCOMP is an interdisciplinary approach to the fabrication and biochemical and biophysical characterisation of three-dimensional hybrid scaffold frameworks with controlled pore-hierarchies for bone-engineering. A key aspect of the project is the study of the hierarchical structure of such frameworks by means of 3D X-ray synchrotron imaging techniques.

Polymeric scaffolds suc as Si-hydroxyapatite (SiHA) and Sr-hydroxyapatite (SrHA) are considered promising minerals for bone regeneration as they affect the expression of several genes involved in cell morphogenesis. Thanks to their properties HA, SiHA and SrHA have been identified embedded in the PCL polymer matrix to have a positive influence on bone remodeling during prosthesis implantation. A comparative study was performed on the synthesized scaffolds to investigate the influence of the mineral particles on the scaffold morphology. 

The results of the 3D laminography show that most of the micro-particles are encapsulated in the polymer structure of the hybrid scaffolds, leading to the formation of distorted, patch-like polymer fibers.

 

Project Partners

The project is coordinated by the National Research Tomsk Polytechnic University (Tomsk, Russia) in collaboration with the following partners: Ghent University (Ghent, Belgium), Fraunhofer Institute for Interfacial Engineering and Biotechnology (Stuttgart), Karlsruhe Institute of Technology (Karlsruhe), University Politehnica Bucharest (Bucharest, Romania), National R&D Institute for Optoelectronics (Bucharest, Romania).

Link to project homepage

 

Publications
  1. High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels, Gorodzha, S.; Douglas, T. E. L.; Samal, S. K.; Detsch, R.; Cholewa-Kowalska, K.; Braeckmans, K.; Boccaccini, A. R.; Skirtach, A. G.; Weinhardt, V.; Baumbach, T.; Surmeneva, M. A.; Surmenev, R. A., 2016. Journal of biomedical materials research / A, 104 (5), 1194–1201. doi:10.1002/jbm.a.35642
     
  2. Hybrid biocomposites based on titania nanotubes and a hydroxyapatite coating deposited by RF-magnetron sputtering : Surface topography, structure, and mechanical properties, Chernozem, R. V.; Surmeneva, M. A.; Krause, B.; Baumbach, T.; Ignatov, V. P.; Tyurin, A. I.; Loza, K.; Epple, M.; Surmenev, R. A.2017. Applied surface science, 426, 229–237. doi:10.1016/j.apsusc.2017.07.199
     

  3. RF magnetron sputtering of a hydroxyapatite target: A comparison study on polytetrafluorethylene and titanium substrates, Surmenev, R. A.; Surmeneva, M. A.; Grubova, I. Y.; Chernozem, R. V.; Krause, B.; Baumbach, T.; Loza, K.; Epple, M., 2017. Applied surface science, 414, 335 –344. doi:10.1016/j.apsusc.2017.04.090

  4. Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration, Surmenev, R. A.; Shkarina, S.; Syromotina, D. S.; Melnik, E. V.; Shkarin, R.; Selezneva, I. I.; Ermakov, A. M.; Ivlev, S. I.; Cecilia, A.; Weinhardt, V.; Baumbach, T.; Rijavec, T.; Lapanje, A.; Chaikina, M. V.; Surmeneva, M. A., 2019. European polymer journal, 113, 67–7. doi:10.1016/j.eurpolymj.2019.01.042
     
  5. Low-temperature argon and ammonia plasma treatment of poly-3-hydroxybutyrate films: Surface topography and chemistry changes affect fibroblast cells in vitro, Surmenev, R. A.; Chernozem, R. V.; Syromotina, D. S.; Oehr, C.; Baumbach, T.; Krause, B.; Boyandin, A. N.; Dvoinina, L. M.; Volova, T. G.; Surmeneva, M. A., 2019. European polymer journal, 112, 137–145. doi:10.1016/j.eurpolymj.2018.12.040
     
  6. Effect of low-temperature plasma treatment of electrospun polycaprolactone fibrous scaffolds on calcium carbonate mineralisation, Ivanova, A. A.; Syromotina, D. S.; Shkarina, S. N.; Shkarin, R.; Cecilia, A.; Weinhardt, V.; Baumbach, T.; Saveleva, M. S.; Gorin, D. A.; Douglas, T. E. L.; Parakhonskiy, B. V.; Skirtach, A. G.; Cools, P.; De Geyter, N.; Morent, R.; Oehr, C.; Surmeneva, M. A.; Surmenev, R. A., 2018. RSC Advances, 8 (68), 39106–39114. doi:10.1039/c8ra07386d
     
  7. 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study, Shkarina, S.; Shkarin, R.; Weinhardt, V.; Melnik, E.; Vacun, G.; Kluger, P.; Loza, K.; Epple, M.; Ivlev, S. I.; Baumbach, T.; Surmeneva, M. A.; Surmenev, R. A., 2018. Scientific reports, 8 (1), Article: 8907. doi:10.1038/s41598-018-27097-7
     
  8. In vitro degradation behaviour of hybrid electrospun scaffolds of polycaprolactone and strontium-containing hydroxyapatite microparticles, Melnik, E. V.; Shkarina, S. N.; Ivlev, S. I.; Weinhardt, V.; Baumbach, T.; Chaikina, M. V.; Surmeneva, M. A.; Surmenev, R. A., 2019. Polymer degradation and stability, 167, 21– 32. doi:10.1016/j.polymdegradstab.2019.06.017
     
  9. Characterization of biomimetic silicate- and strontium-containing hydroxyapatite microparticles embedded in biodegradable electrospun polycaprolactone scaffolds for bone regeneration, Surmenev, R. A.; Shkarina, S.; Syromotina, D. S.; Melnik, E. V.; Shkarin, R.; Selezneva, I. I.; Ermakov, A. M.; Ivlev, S. I.; Cecilia, A.; Weinhardt, V.; Baumbach, T.; Rijavec, T.; Lapanje, A.; Chaikina, M. V.; Surmeneva, M. A., 2019. European polymer journal, 113, 67–77. doi:10.1016/j.eurpolymj.2019.01.042